Membrane-elution analysis of content of cyclins A, B1, and E during the unperturbed mammalian cell cycle.(Research)(Report)

Cell Division | September 24, 2007| | Copyright

Authors: Stephen Cooper (corresponding author) [1]; Michelle Paulsen [2]; Mats Ljungman [2]; Dang Vu-Phan [1]; Duyang Kim [1]; Mariam Gonzalez-Hernandez [1]

Background

We describe an automated approach that easily yields cells in all phases of the mammalian cell cycle. Further, we apply this method to the measurement of cyclin contents during the normal mammalian cell cycle. The automated membrane-elution method (colloquially referred to as the "baby machine") allows numerous replications of cell-cycle analyses without long-term, ...

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