Testing a non-lethal DNA isolation technique for freshwater mussels that is compatible with polymerase chain reaction (PCR) based studies.(deoxyribonucleic acid)

From: Transactions of the Kansas Academy of Science | Date: March 22, 2007| Author: Spicer, Judson F., Jr.; Sobieski, Rodney J.; Crupper, Scott S. | Copyright information

In this study, a non-lethal DNA isolation technique was developed for freshwater mussels. A total of 45 Quadrula quadrula, 21 Q. metanevra, and 19 Q. pustulosa were collected from the Neosho and Verdigris Rivers in eastern Kansas. DNA was successfully isolated from 82 mussels using the non-lethal technique developed in this study. Spectrophotometer analysis of the 82 DNA samples resulted in an average DNA concentration of 488 ng/[micro]l. Agarose gel electrophoresis of polymerase c...

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