Fluorometric measurement of oxidative burst in lobster hemocytes and inhibiting effect of pathogenic bacteria and hypoxia.

From: Journal of Shellfish Research | Date: December 1, 2006| Author: Moss, Brandon; Allam, Bassem | Copyright information

ABSTRACT The stimulation of hemocytes during phagocytosis leads to the generation of a series of oxygen radicals known as reactive oxygen species (ROS). Among these, hydrogen peroxide plays an important microbicidal role by directly killing microorganisms or by serving as an intermediate for other antimicrobial radicals. In this study, we adapted a technique using 2',7'-dichlorofluorescein-diacetate (DCFH-DA) to measure [H.sub.2][O.sub.2] production in lobster hemocytes. After oxid...

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