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Nitrogen dioxide-induced reactive oxygen intermediates production by human alveolar macrophages and peripheral blood mononuclear cells.
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Alveolar macrophages located on the alveolar surface have contact with air pollutants. We evaluated the dose-dependent effect of nitrogen dioxide exposure on the oxidative metabolism of alveolar macrophages and peripheral blood mononuclear cells by measuring the spontaneous and stimulated reactive oxygen intermediates production. Alveolar macrophages or peripheral blood mononuclear cells were placed on a polycarbonate membrane, which was in direct contact with the surface of a nutrient reservoir. The cells were exposed to nitrogen dioxide during different periods of time, varying between 30 ...
<.005; Fig. 3). An extended exposure time for 0.1 ppm up to 120 min yielded nearly constant values (60 min, 1.7-fold; 120 min, 1.9-fold; Fig. 2). Production of ROIs leveled off at exposure times of up to 120 min for 0.5 ppm [NO.sub.2] (60 and 120 min, 2.2-fold). After an exposure time of 120 min, all tested [NO.sub.2] concentrations yielded a similar ROIs production of about 1.9- to 2.2-fold, compared with standard air (r = 0.48, p>
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