Separation of guanine and hypoxanthine with some ionic liquids in RP-HPLC.(reversed phase high performance liquid chromatography)

American Journal of Applied Sciences | December 1, 2006| | Copyright

Abstract: In this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RPHPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][B[F.sub.4]]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][B[F.sub.4]]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-octyl-3-methylimidazolium methylsulfate ([OMIm][MS]) were used. Guanine and hypoxanthine couldn't be separated with many different kinds of unadjusted mobile ...

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