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Rapid antibody purification using ultrafiltration & centrifugal affinity columns: the filtration-based method for the rapid purification of monoclonal antibodies is completed in less than two hours.(Application Note)
From:
Bioscience Technology
| Date:
July 1, 2006| Author:
Barbagallo, Christopher; Koticha, Darshan; Rapiejko, Peter
| COPYRIGHT 2006 Advantage Business Media. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group.Copyright information
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Monoclonal antibodies are important research tools and potent therapeutic agents for many different cancers. (1,2) Purification of these proteins, on the laboratory scale, is a time-consuming process. Traditionally, antibodies from hybridomas have been purified using a complex method that involves an ammonium sulfate precipitation, dialysis and protein-A/G affinity chromatography. (3,4) The entire process typically requires several days to complete and can be particularly challengi...
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Rapid antibody purification using ultrafiltration & centrifugal affinity columns: the filtration-based method for the rapid purification of monoclonal antibodies is completed in less than two hours.(Application Note)
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; Monoclonal antibodies are important research tools and potent therapeutic agents for many different cancers. (1,2) Purification of these proteins, on the laboratory scale, is a time-consuming process. Traditionally, antibodies from hybridomas have been purified using a complex method that involves
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