SARS-associated coronavirus replication in cell lines.(RESEARCH)

From: Emerging Infectious Diseases | Date: January 1, 2006| Author: Birch, Chris; Catton, Mike; Chibo, Doris; Druce, Julian; Kaye, Matthew; Kostecki, Renata; Morris, Jessica; Tran, Thomas | Copyright information

Given the potential fer laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also u...

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