Histidine-tagged protein purification using recombinant DNA techniques.(APPLICATION Note)

Bioscience Technology | November 1, 2005| | Copyright

The use of recombinant DNA techniques for protein expression and purification has increased greatly in recent years. The abundant amounts of recombinant proteins that can be produced in bacterial hosts gives rDNA (recombinant DNA) techniques an advantage in comparison to the use of native sources. Many DNA plasmid vectors include an attached fusion tag sequence, intended to simplify the subsequent protein purification step. Although many different fusion tag systems are available, the first choice for most scientists is the polyhistidine tag. The polyhistidine tag is ...

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