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Cryptococcus gattii in AIDS patients, Southern California.(RESEARCH)

From: Emerging Infectious Diseases  |  Date: 11/1/2005  |  Author: Chaturvedi, Sudha; Chaturvedi, Vishnu; Dyavaiah, Madhu; Larsen, Robert A.

Cryptococcus isolates from AIDS patients in southern California were characterized by molecular analyses. Pheromone MF[alpha]1 and MF[alpha]1 gene fragments were polymerase chain reaction-amplified with fluorescently labeled primers and analyzed by capillary electrophoresis (CE) on DNA analyzer. CE-fragment-length analyses (CE-FLAs) and CE-single-strand conformation polymorphisms (CE-SSCPs) were used to determine Cryptococcus gattii (Cg), C. neoformans (Cn) varieties neoformans ...

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