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Pyrosequencing Bacillus anthracis.
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Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis. In addition, 1 segment in each of the B. anthracis plasmids pXO1 and pXO2 was analyzed to determine ...
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Evaluation and validation of a real-time polymerase chain reaction assay for rapid identification of Bacillus anthracis. (Letters).(Letter to the Editor)
Magazine article from: Emerging Infectious Diseases
; ...detection and identification of Bacillus anthracis. This PCR assay was successfully used...environmental samples for the presence of B. anthracis throughout the 2001 anthrax outbreak...setting, a PCR assay was used to detect B. anthracis directly from clinical specimens, consequently...
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Variance in Anthrax Strains Could Crack Case
Newspaper article from: The Washington Post
; ...different parts of the world, Bacillus anthracis has seemed stuck in its ways. Until recently...to many strains and sub-strains of B. anthracis. And as researchers learn precisely how...academic and arcane goal of understanding B. anthracis genetics. Now those two previously low-profi...
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Sequencing of 16S rRNA Gene: a rapid tool for identification of Bacillus anthracis. (Bioterrorism-Related Antrax).
Magazine article from: Emerging Infectious Diseases
; ...needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming...positions (0.06% to 0.5%). All 86 B. anthracis had an identical 16S gene sequence, designated...association of a distinct 16S sequence with B. anthracis. Consequently, we were able to rapidly...
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Pyrosequencing Technology as a Method for the Diagnosis of Multiple Endocrine Neoplasia Type 2
Magazine article from: Clinical Chemistry
; ...620, 630, 634, 768, 804, and 918 were analyzed by Pyrosequencing technology to match the original analysis of each patient. Template...of unknown significance were analyzed. Conclusion: Pyrosequencing technology offers an accurate, nonisotopic, simple, and rapid...
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Pyrosequencing protocols.(Brief Article)(Book Review)
Magazine article from: SciTech Book News
; ...Medicine, St. Louis) compiles 14 chapters that describe detailed protocols for the multidisciplinary application of Pyrosequencing technology for DNA sequencing, along with an overview of its origins and methodology, and information on techniques, primer selection...
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Ruling out Bacillus anthracis.(Dispatches)
Magazine article from: Emerging Infectious Diseases
; Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround...workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse...
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Update: Investigation of Bioterrorism-Related Inhalational Anthrax - Connecticut, 2001.
Newspaper article from: Morbidity and Mortality Weekly Report
; ...identify the source of exposure to Bacillus anthracis continue for a case of inhalational anthrax...is continuing in persons exposed to B. anthracis, and surveillance to detect new cases...20, the isolate was identified as B. anthracis at the CDPH laboratory with confirmation...
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Update: Investigation of Anthrax Associated with Intentional Exposure and Interim Public Health Guidelines, October 2001.
Newspaper article from: Morbidity and Mortality Weekly Report
; ...intentional release of Bacillus anthracis. This report also includes interim...laboratory confirmed by isolation of B. anthracis from an affected tissue or site or 2) other laboratory evidence of B. anthracis infection based on at least two...
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Viral enzyme detects, kills bacterium. (Anthrax Stopper).(Brief Article)
Magazine article from: Science News
; ...innovative way to detect and kill Bacillus anthracis, the bacterium that causes anthrax. Seeking...infected with a bacterial relative of B. anthracis, Raymond Schuch of Rockefeller University...antibiotics can treat many strains of B. anthracis, there's concern that resistant strains...
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Comparative evaluation of protective antigen produced from Bacillus anthracis & Escherichia coli
Magazine article from: Indian Journal of Medical Research
; ...Protective antigen was isolated and purified from the Sterne strain of Bacillus anthracis, B. anthracis lacking pXO1 and pXO2 transformed with pYS5 (B. anthracis pYS5) and recombinant Escherichia coli transformed with pQE30 containing PA gene...
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