Quantitate PARP activity in cell extracts and determine PARP inhibitor efficacy.(Poly(ADP-ribose) polymerase1) From: Bioscience Technology | Date: July 1, 2005| Author: Bazar, Leonard S. | COPYRIGHT 2005 Advantage Business Media. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group.Copyright information

This assay measures the poly(ADP) ribosylation of histones precoated on 96-well plates using biotinylated NAD as substrate. Coupled with streptavidinhorseradish peroxidase (Strep-HRP), a colorimetric or chemiluminescent readout provides a rapid and accurate assessment of the level of PARP activity in cell extracts and for screening PARP inhibitors.

Poly(ADP-ribose) polymerase1 (PARP-1) is a nuclear enzyme activated by DNA breaks. Activated PARP-1 cleaves NAD into nicotin...

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