Gram stains from EDTA tubes.(Answering Your Questions)

From: Medical Laboratory Observer | Date: July 1, 2004| Author: | Copyright information

Q Do you have any references that address the issue of using specimens that are received in sterile vacutainer tubes with EDTA additive (liquid or dry) for Gram-stain examinations? I know that EDTA and other additives can be toxic to bacterial specimens placed in this type of container and are, therefore, not suitable for culturing; however, I do not know of a reference that indicates that it is not suited for holding specimens for Gram staining. Will the EDTA cause the bacteria to lyse if it is placed in this medium? On rare occasions, I have examined peripheral smears from EDTA ...

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