Voltage-dependent [Ca.sup.2+] fluxes in skeletal myotubes determined using a removal model analysis.

The Journal of General Psychology | January 1, 2004| | Copyright

INTRODUCTION

Contractile activation in muscle is initiated by a massive efflux of [Ca.sup.2+] ions from the SR. This [Ca.sup.2+] flux is tightly controlled by the plasma membrane voltage (Melzer et al., 1995; Dirksen, 2002; see Fig. 2 A). In experiments on isolated muscle cells, SR [Ca.sup.2+] release can be reproducibly triggered by plasma membrane depolarization. The precise synchronization with depolarization permits to study intracellular [Ca.sup.2+] release in muscle cells in better kinetic detail than in any other cellular system. The release flux of ...

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