Market profile: amino acid analyzers.

Instrument Business Outlook | April 15, 2003 | Copyright

In the traditional method for breaking down proteins for amino acid analysis, the protein or peptide sample is first hydrolyzed in a vacuum or inert atmosphere. A stepped gradient of buffers elutes the sample (modern analyzers make use of a continuous gradient) which then passes through a resin-packed ion-exchange column. The reagent ninhydrin is applied and the resulting reaction turns the primary amino acids a bluish color and the secondary amino acids brownish-yellow.

The ramifications of this automated technique, which was first pioneered by Nobel laureates ...

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